plot 12.0 enzyme kinetics module 1.3 Search Results


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DLC showed anti-inflammatory properties in vitro. The H 2 O 2 group was established in vivo. a <t>iNOS/Arg-1</t> fluorescence double-staining was used to analyze the anti-inflammatory properties of PLA/DLC. b , c The mRNA expression levels of IL-6 and TNF- α were detected by real-time PCR. d , e The concentrations of TNF- α and IL-6 in cell supernatant were measured by ELISA. Data represent independent experiments, and all data are given as mean ± SD; NS non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001
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DLC showed anti-inflammatory properties in vitro. The H 2 O 2 group was established in vivo. a <t>iNOS/Arg-1</t> fluorescence double-staining was used to analyze the anti-inflammatory properties of PLA/DLC. b , c The mRNA expression levels of IL-6 and TNF- α were detected by real-time PCR. d , e The concentrations of TNF- α and IL-6 in cell supernatant were measured by ELISA. Data represent independent experiments, and all data are given as mean ± SD; NS non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001
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DLC showed anti-inflammatory properties in vitro. The H 2 O 2 group was established in vivo. a <t>iNOS/Arg-1</t> fluorescence double-staining was used to analyze the anti-inflammatory properties of PLA/DLC. b , c The mRNA expression levels of IL-6 and TNF- α were detected by real-time PCR. d , e The concentrations of TNF- α and IL-6 in cell supernatant were measured by ELISA. Data represent independent experiments, and all data are given as mean ± SD; NS non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001
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Cayman Chemical nitrate reductase enzyme
DLC showed anti-inflammatory properties in vitro. The H 2 O 2 group was established in vivo. a <t>iNOS/Arg-1</t> fluorescence double-staining was used to analyze the anti-inflammatory properties of PLA/DLC. b , c The mRNA expression levels of IL-6 and TNF- α were detected by real-time PCR. d , e The concentrations of TNF- α and IL-6 in cell supernatant were measured by ELISA. Data represent independent experiments, and all data are given as mean ± SD; NS non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001
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DLC showed anti-inflammatory properties in vitro. The H 2 O 2 group was established in vivo. a <t>iNOS/Arg-1</t> fluorescence double-staining was used to analyze the anti-inflammatory properties of PLA/DLC. b , c The mRNA expression levels of IL-6 and TNF- α were detected by real-time PCR. d , e The concentrations of TNF- α and IL-6 in cell supernatant were measured by ELISA. Data represent independent experiments, and all data are given as mean ± SD; NS non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001
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Galectin Therapeutics galectin-9 antibody
DLC showed anti-inflammatory properties in vitro. The H 2 O 2 group was established in vivo. a <t>iNOS/Arg-1</t> fluorescence double-staining was used to analyze the anti-inflammatory properties of PLA/DLC. b , c The mRNA expression levels of IL-6 and TNF- α were detected by real-time PCR. d , e The concentrations of TNF- α and IL-6 in cell supernatant were measured by ELISA. Data represent independent experiments, and all data are given as mean ± SD; NS non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001
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Cayman Chemical cofactor preparation (13 µl)
DLC showed anti-inflammatory properties in vitro. The H 2 O 2 group was established in vivo. a <t>iNOS/Arg-1</t> fluorescence double-staining was used to analyze the anti-inflammatory properties of PLA/DLC. b , c The mRNA expression levels of IL-6 and TNF- α were detected by real-time PCR. d , e The concentrations of TNF- α and IL-6 in cell supernatant were measured by ELISA. Data represent independent experiments, and all data are given as mean ± SD; NS non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001
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Boster Bio interleukin 13
DLC showed anti-inflammatory properties in vitro. The H 2 O 2 group was established in vivo. a <t>iNOS/Arg-1</t> fluorescence double-staining was used to analyze the anti-inflammatory properties of PLA/DLC. b , c The mRNA expression levels of IL-6 and TNF- α were detected by real-time PCR. d , e The concentrations of TNF- α and IL-6 in cell supernatant were measured by ELISA. Data represent independent experiments, and all data are given as mean ± SD; NS non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001
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DLC showed anti-inflammatory properties in vitro. The H 2 O 2 group was established in vivo. a iNOS/Arg-1 fluorescence double-staining was used to analyze the anti-inflammatory properties of PLA/DLC. b , c The mRNA expression levels of IL-6 and TNF- α were detected by real-time PCR. d , e The concentrations of TNF- α and IL-6 in cell supernatant were measured by ELISA. Data represent independent experiments, and all data are given as mean ± SD; NS non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001

Journal: Nano-Micro Letters

Article Title: Diamond-Like Carbon Depositing on the Surface of Polylactide Membrane for Prevention of Adhesion Formation During Tendon Repair

doi: 10.1007/s40820-024-01392-7

Figure Lengend Snippet: DLC showed anti-inflammatory properties in vitro. The H 2 O 2 group was established in vivo. a iNOS/Arg-1 fluorescence double-staining was used to analyze the anti-inflammatory properties of PLA/DLC. b , c The mRNA expression levels of IL-6 and TNF- α were detected by real-time PCR. d , e The concentrations of TNF- α and IL-6 in cell supernatant were measured by ELISA. Data represent independent experiments, and all data are given as mean ± SD; NS non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001

Article Snippet: RAW264.7 cells were cultured on PLA or PLA/DLC membranes in 24-well grew for 1 day to form a confluent monolayer and incubated with H 2 O 2 (100 μM) for 24 h. Cells were fixed with 4% paraformaldehyde for 20 min, permeabilized with 0.5% Triton × 100 in PBS, blocked with 5% BSA, and then incubated with Arg-1 (1:100 dilution, Arginase-1, Rabbit mAb #93,668, CST, USA) and iNOS (1:100 dilution, Rabbit mAb #13,120, CST, USA) antibodies at 4 °C overnight.

Techniques: In Vitro, In Vivo, Fluorescence, Double Staining, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay