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Image Search Results
Journal: Nano-Micro Letters
Article Title: Diamond-Like Carbon Depositing on the Surface of Polylactide Membrane for Prevention of Adhesion Formation During Tendon Repair
doi: 10.1007/s40820-024-01392-7
Figure Lengend Snippet: DLC showed anti-inflammatory properties in vitro. The H 2 O 2 group was established in vivo. a iNOS/Arg-1 fluorescence double-staining was used to analyze the anti-inflammatory properties of PLA/DLC. b , c The mRNA expression levels of IL-6 and TNF- α were detected by real-time PCR. d , e The concentrations of TNF- α and IL-6 in cell supernatant were measured by ELISA. Data represent independent experiments, and all data are given as mean ± SD; NS non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001
Article Snippet: RAW264.7 cells were cultured on PLA or PLA/DLC membranes in 24-well grew for 1 day to form a confluent monolayer and incubated with H 2 O 2 (100 μM) for 24 h. Cells were fixed with 4% paraformaldehyde for 20 min, permeabilized with 0.5% Triton × 100 in PBS, blocked with 5% BSA, and then incubated with Arg-1 (1:100 dilution, Arginase-1, Rabbit mAb #93,668, CST, USA) and
Techniques: In Vitro, In Vivo, Fluorescence, Double Staining, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay